FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbq50k-2.351000000e808f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbq50k-2.351000000e808f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8968305
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG13350.018.6689433
CCTCTAG19200.017.70521
TACCGCC17600.016.98540319
GACCTCG10150.016.60865825
AATACCG19150.016.31584417
TAGACTA18600.016.3138989
ACCGCCA18550.016.236720
GATAGAC20050.015.4704027
AGGTTAG13100.015.44224838
GCGACCT11150.014.91750323
AGGAACC14850.014.8332577
GACATCC14400.014.82852658
GGAACCT14950.014.583698
CAGGTTA14200.014.40430637
CCGCCAA21100.014.27452521
TGCGACC12400.014.13878922
CGACCTC12100.014.11781624
TATACAC127000.014.07263345
GGAGACC12750.013.75066525
GGGAGAC12450.013.72092924
Produced by FastQC (version 0.11.2)