FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbq50k-1.351000000e8065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbq50k-1.351000000e8065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5139844
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG1202.5300142E-718.7293435
TACACCG7100.017.4103765
ACACCGG6200.016.3126546
CCTCTAG8700.015.8666841
GGCTTAC7900.015.4682881
CTAGAGG6750.015.08343
GAGACCC5550.014.57851726
GGGAGAC5500.014.30254824
GATAGAC9250.014.09256657
ACCGTCG7550.013.9911929
GGAACCT7300.013.8545838
CGTCGTT7600.013.64070611
TAGACTA9900.013.6213429
GACCTCG5450.013.60897725
TTGCGAC5300.013.57007221
TAGGACG6400.013.3446585
GGAGACC5750.013.28982125
ACCGCCA9500.013.24867520
TGCGACC5600.013.24445122
TATACAC69600.013.24228645
Produced by FastQC (version 0.11.2)