FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbk50r54_abnt-3.351000000e81de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbk50r54_abnt-3.351000000e81de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8764138
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG11700.016.3347455
TAGACTA13750.014.8805179
GATAGAC15550.014.1701697
TATACAC112900.014.10143245
CCTCTAG15850.013.8309651
GGTTAGA14600.012.6931961
AATACCG16050.012.46849617
CTTACAC25600.012.3565853
CTAGAGG12000.012.2389043
TAGGACG10950.012.1148265
TTATACA168200.011.9384945
GTAATAC19900.011.9219313
TATACTG22500.011.8916955
GTACTAG16500.011.7794371
ACCGCCA17500.011.69190120
TAATACG14700.011.6817174
GTTAGAC12250.011.6202342
CCTAGAG16800.011.50988523
GGCTTAC14150.011.4996871
CCGCCAA18300.011.42651121
Produced by FastQC (version 0.11.2)