FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbk50r54_abnt-2.351000000e81c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbk50r54_abnt-2.351000000e81c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10608160
Sequences flagged as poor quality0
Sequence length51
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG17200.019.6247433
GGAACCT18900.017.6018128
TGCGACC12350.017.47285322
TTGCGAC13050.017.0523521
GCGACCT13200.016.85857423
AGGAACC20850.016.8180667
ATCCACG21100.015.87757430
GATGGAC21000.015.41347936
TCCACGA21700.015.12771931
ACGGACT10800.014.98546927
GATAGAC18150.014.9853287
CCACGAT22300.014.92234932
CACGATG22200.014.88828633
ATGGACC22150.014.81619437
CGACCTC16100.014.79924424
ATTGCGA15800.014.79563820
GACCTCG15350.014.64371225
ACGATGG21200.014.63596934
GTGTAAG33650.014.6130371
CCTCTAG19700.014.6084221
Produced by FastQC (version 0.11.2)