FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbk50r54_abnt-1.351000000e81a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbk50r54_abnt-1.351000000e81a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10950857
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC154140.14075610703344954RNA PCR Primer, Index 27 (95% over 21bp)
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG148120.1352588203827335No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG135560.12378939840050873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC31450.032.74448815
CCACGAG32150.032.57920
GACGGAC30100.032.1095926
ACGGACT30700.031.84810827
GAGACGG30450.031.66669824
CCGAGCC33100.031.24836714
AGACGGA31700.030.5598225
ACGAGAC34750.030.20611222
GCCCACG34700.029.5371218
TCCGAGC35450.029.3040313
CGTATGC35150.028.77754241
TCGTATG34850.028.70276340
TATGCCG34000.028.6930943
TGCCGTC33250.028.46144545
CTCGTAT35200.028.4152939
GTATGCC34750.028.20318642
CTCCGAG37050.028.03853812
TATCTCG30550.027.88449336
TCTCGTA31650.027.48349838
ATGCCGT34800.027.3229544
Produced by FastQC (version 0.11.2)