FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbk50r54-3.351000000e8121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbk50r54-3.351000000e8121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13566887
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG25450.016.7812441
CTAGAGG19500.016.5685313
TAGACTA25800.015.5124739
TACCGCC26750.015.12972619
GATAGAC25750.015.1060057
AATACCG26650.014.68109317
CCGCCAA27250.014.60463521
TACACCG19150.014.5590725
GGAACCT21250.014.2841898
CGACCTC15650.014.07977524
GACCTCG15500.014.07091725
CACGATG21350.013.90498333
ACCGCCA28300.013.90381520
AGGAACC22000.013.899437
GCGACCT15800.013.51918423
GACATCC20500.013.49062258
ATCCTAC20600.013.42632242
TATACAC191800.013.11899845
TGCGACC16300.012.82860122
ATCCACG23250.012.67192930
Produced by FastQC (version 0.11.2)