FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbk50r54-2.351000000e80f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbk50r54-2.351000000e80f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7334924
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG10300.013.7480215
CCTCTAG10450.013.2318061
CTAGAGG8500.012.2639683
CTCCGAG15500.011.74720745
GACCGTG3501.382432E-1011.5595637
TCCGAGC10100.011.35089545
ACCGTGT4850.011.12260158
GACCTCG6550.010.98124325
TAGACTA9850.010.9532239
ACACCGT7200.010.9262846
ACCGCGT2707.996932E-710.8222238
TATACAC115700.010.80246245
TACCCCG3602.7066562E-910.6141045
GATAGAC11650.010.6114247
GTTCTAG7300.010.5575141
ACACCGG8100.010.5447316
TCTACAC13350.010.5245263
GTGCTAG6900.010.5125111
GAGACCC6850.010.50031326
TAGGACG7350.010.397495
Produced by FastQC (version 0.11.2)