FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbk50r54-1.351000000e80df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbk50r54-1.351000000e80df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2711419
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG44460.16397318157024054No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG34030.12550623861527857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG2750.016.3472485
TACACAT34800.013.1125845
GCTACAC4100.012.69051553
TATACTG8700.012.6597225
CTAGAGG3601.8189894E-1212.5679023
CTTACAC6700.012.4928693
TAGACCG1454.893419E-412.40136055
CCTCTAG3651.8189894E-1212.3978091
TATACAC65350.012.24547945
GTGTAAG6000.012.0672011
GACAGTC2452.2723907E-711.9268187
CCTATAC4000.011.8766683
GTGCTAG3459.640644E-1111.8048711
AGACCTA3052.2937456E-911.7914576
GTAGCAC2901.247281E-811.7011513
GTCCAGA4101.8189894E-1211.5889281
GCCTAGA2553.505338E-711.5348241
TTATACA93150.011.51083745
CTATACT8950.011.3743024
ACCCCGG1400.00504487311.3130011
Produced by FastQC (version 0.11.2)