FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbabrh-2.351000000e8164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbabrh-2.351000000e8164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9053735
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG116530.1287093116818639No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG104950.11591901022064374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG11800.013.715325
CCTCTAG14000.013.4347211
GATAGAC13550.012.6075227
CTACACT15250.011.88474754
CTAGAGG10750.011.5910843
TATACAC121550.011.42994445
GTTAGAC11300.011.4278952
TAGACAG8450.011.1724415
TCCGAGC11600.011.04657145
CTTACAC24350.010.9787483
GGTTAGA14150.010.890081
CCGGTCG8850.010.6674719
ACCCGGT8650.010.6542577
CCCCCCA11200.010.63685627
TAGACTA15850.010.6362239
ACACCGG10050.010.5120466
TTATACA172800.010.31668245
TAAGACC14350.010.2619834
TGTACCG8600.010.19345955
CTATACT28800.010.1476874
Produced by FastQC (version 0.11.2)