FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azbabrh-1.351000000e814b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azbabrh-1.351000000e814b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16033987
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG389330.2428154644256603No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG357800.22315098546606033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG23150.012.90361
GTGTAAG40800.012.64627551
GTCTAGG10050.011.9343221
TATACAC227100.011.7090145
TATACTG36100.011.7050545
TACACTG28200.011.4772055
TACACAG30550.011.4036335
CTATACT40800.011.2575374
GTCCTAG8450.011.2481361
CTTACAC42550.011.2199363
TACACCG20250.011.2103495
TAGACAG18150.011.145225
GTATTAG44750.010.8725991
TACACAT133700.010.70928845
AGCACTG51400.010.5384495
GTAATAC33750.010.3241543
GGTTAGA25300.010.2864451
TATACAG32450.010.2510835
TAGGACG15900.010.1778995
GATAGAC23200.010.1724167
Produced by FastQC (version 0.11.2)