FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azb-3.351000000e804c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azb-3.351000000e804c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6996555
Sequences flagged as poor quality0
Sequence length51
%GC30

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT5300.023.75755923
TAAGGCG5400.023.74009929
GACTAAG6150.020.839626
ACGAGAC6050.020.81240722
CCACGAG6300.020.70032120
TATGCCG6550.019.91179743
CGAGCCC6150.019.76792315
GAGACTA6550.019.22367324
CCGCCAA13450.018.72342921
GCGACCT6850.018.71000723
TAGACTA14450.018.516929
TGCCGTC6850.018.38320545
CCTCTAG14400.018.359721
AATACCG13400.018.29081517
ACCGCCA13050.018.26354220
GCGAATC6250.017.99262433
GCCCACG7000.017.66665518
TACCGCC13650.017.62547319
GTATGCC7400.017.62463242
ATGCCGT7200.017.48957844