FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azb-2.351000000e8022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azb-2.351000000e8022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8163604
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAG6250.019.42045626
CGAGACT6150.019.00537923
CTAGAGG12250.018.1267853
CCACGAG6600.018.05012520
ACGAGAC6600.017.70955722
GGAACCT12100.017.4617658
CGACCTC8900.017.4263924
CGAGCCC7200.017.19507415
AGGAACC13000.016.9444857
CCTCTAG14450.016.3118631
GCCCACG7500.016.18381118
GCGACCT9500.016.08916923
CCGAGCC7850.016.0580314
TTGCGAC9550.015.76956721
GACCTCG9550.015.7694725
TAAGGCG7600.015.38434729
TGCGACC9600.015.219151522
GATAGAC14850.014.9849187
ACGATGG14250.014.83208834
GAGACTA8650.014.81177324