FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_azb-1.351000000e8008.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_azb-1.351000000e8008.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402114
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT8350.032.03299323
CCACGAG9400.028.21572320
GACTAAG9350.027.40481826
TAAGGCG9200.027.3704929
TATGCCG9750.027.20476343
ACGAGAC9850.026.69848622
CGAGCCC9750.026.57954815
TCGTATG10350.026.49641240
GCGAATC8450.025.54270733
GTATGCC10750.025.51049842
GAGACTA10350.025.1915424
TGCCGTC10400.025.07218645
GCCCACG10900.024.53884518
CCGAGCC10600.024.44817214
ATGCCGT11000.024.11331444
CGTATGC11450.023.95090541
TCCGAGC10800.023.78677213
CGAATCT9600.022.95129634
AAGGCGA11250.022.58282730
TCTCGTA11200.022.07538838
Produced by FastQC (version 0.11.2)