FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_ab-3.351000000e823d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_ab-3.351000000e823d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11135343
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAG17150.017.2620221
GACTAGG6650.017.24382626
TAGACTA18750.015.3494169
ACTAGGC7300.015.09240127
CGAGACT8000.014.61498723
GATAGAC20550.013.6767017
CTAGAGG15650.013.5698353
GACCTCG13100.013.55941325
TGCGACC13700.013.45793422
GGAACCT15050.013.4458778
CGACCTC13900.013.42605524
TACACCG18100.013.291945
ACACCGG15250.013.2695386
TTGCGAC14250.013.09629121
TAGGACG15350.013.03661255
CGAGCCC8850.012.97353615
GCGACCT14050.012.80261823
TAGTACG16450.012.71268445
CCACGAG9700.012.28534420
GAGACTA9700.012.05359824