FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_ab-2.351000000e8213.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_ab-2.351000000e8213.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13259965
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC162400.012.01555745
TACACTG21050.011.4257475
CTAGAGG18600.011.3263453
CTACACT25650.011.3042674
TATACTG41300.011.157245
TACACAG28600.011.0030975
TACACCG19400.010.8912835
GTGTAAG31350.010.697021
TAGACAG15850.010.35253055
CCTCTAG19250.010.0052241
CTTACAC31550.09.9083083
TACACAT91700.09.90426545
GTATTAG45250.09.8647541
TTATACA232000.09.70934645
GGACCGT7050.09.56500156
ACAGTGC21750.09.5078328
GTACTAG21050.09.47261
ACACTGC26350.09.4688076
CTAGCAC15250.09.358163
CTATACT50850.09.3105454