FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l02n01_ab-1.351000000e81f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l02n01_ab-1.351000000e81f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921972
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGC1205.225391E-616.8584256
AGGACCG1104.6793284E-516.3475655
GCTAGGG1004.0022872E-415.8346313
CCAGGAC2302.3646862E-1115.736283
CTAAGAC1351.504415E-515.0806033
ACAGGAC2152.395609E-914.729893
CAGGACC3400.014.6370554
CGACCCC1453.135374E-513.9517996
ACTAGGC1301.9433213E-413.83255427
ACGAGAC1801.1358952E-613.73649422
TACACCG2359.156793E-913.3910895
CGAGACT1851.5461646E-613.36523823
TACACGG1352.6755565E-413.3202385
TCCTACG1200.001591565613.1955262
TATACAC23250.013.14938845
GCGGGAG2401.2285454E-813.113131544
CCTAGAG3101.6370905E-1113.05168323
GTATAGG2451.4970283E-812.9292761
ACAGTGC2857.858034E-1012.6191728
GTACTAG3253.274181E-1112.5314521
Produced by FastQC (version 0.11.2)