FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_yw3.352000000e8056.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_yw3.352000000e8056.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4386232
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC114510.26106690206993155No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG102120.232819422228464No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG92710.2113659286604083No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT92090.20995241473775214No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG53700.12242854459134857No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA46010.10489641222808095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG6850.014.7807335
GTCTAGG2652.4556357E-1013.5844851
ACAGTCC3151.8189894E-1213.5713188
TACACAG9000.013.4997365
GTGTTAG7000.013.4995831
GTGTAAG12800.013.3589631
CCTCTAG6400.013.0074111
CCTAGAG6950.012.95027423
GTCTTAT7700.012.8567451
GTCCTAG2309.8627424E-812.7169971
TATACAC85500.012.63104545
TCTAGAC2901.020453E-912.4134093
CTAGAGG6700.012.0891783
TACACAT47050.011.95486145
GTATATA25200.011.7853491
GTGTACG3451.05501385E-1011.7387681
GTATAGG5050.011.58381
GCGACCT7000.011.57199423
TAGACTA6050.011.5288329
ACACCGG6000.011.2497816
Produced by FastQC (version 0.11.2)