FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_yw2.352000000e803c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_yw2.352000000e803c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848195
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC28890.10143266173839924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCT3900.021.92470223
CCTAGGG807.569825E-519.6868863
CGACCTC4250.019.58968724
TGCGACC4450.018.70925122
TTGCGAC4500.018.50137121
ATTGCGA4650.018.3884620
GACCTCG4700.017.7140825
CGATGTT5050.016.93105730
CTAGAGG5200.016.4417953
CCCTAGG850.002566947815.8818582
TACCCCG1004.1707262E-415.7495095
ATCGTCC2600.015.57561642
GATTGCG5500.015.546061519
GGACATC4400.015.3401617
TACACCG4700.015.3186715
CCTACAC5900.015.25536813
GGGAGAC3550.015.21239624
GGAGACC3750.015.00111225
GACATCC4550.014.8352228
GTTCTAG2751.8189894E-1214.7268131
Produced by FastQC (version 0.11.2)