FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_yw1.352000000e8012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_yw1.352000000e8012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2952349
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC67070.2271750392653443No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG58570.198384405095739No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG51630.17487769907961423No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT50330.1704744256183805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG3850.016.9476573
CCTCTAG4500.014.4996611
TACCCCG1453.108782E-513.9649555
TAATACG4700.013.8826554
GTGTAAG9050.013.6737151
CCTAGGA1000.0075558713.4996852
GTCTAGC1851.5303704E-613.3780661
CTAACCT4800.013.1246944
CTATACT8000.012.9371984
TATACAC52900.012.71666345
CTACCCT1952.7632705E-612.6920124
GTCTAAC3750.012.5997072
GCTACCC2502.1494998E-812.5997063
CTAAGGT2156.006958E-712.5578464
TAGACAG2704.6857167E-912.4994965
GCTTATA9800.012.397671
ACAGTGC4550.012.3627688
CTTAGAC2552.8334398E-812.3526533
TACACAT28150.012.30835445
GTGCTAC3304.9112714E-1112.2724411
Produced by FastQC (version 0.11.2)