FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_otd-2.352000000e813b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_otd-2.352000000e813b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257542
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC19710.1567343277600271No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG18550.14750998376197375No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT15690.12476720459435947No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG15660.12452864397372017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG550.003936236320.4549981
GGGCCGT706.984799E-419.2853746
GGCCGTG1102.2567037E-618.4087667
GTCCTAG800.001710712816.8753741
GTCCTAT1205.172791E-616.8753721
GTGTAGT2302.5465852E-1115.652521
GTCCATA1502.5733752E-615.0003321
TATACTA5350.014.7193665
CTGACGC3100.014.51471845
GTGTAAG3600.014.3753181
CTATACT3950.014.2402544
TACACCG1901.3324279E-714.2102755
TCTGACG3101.8189894E-1213.78898244
AGGCCCC1150.001202048513.695416
ACACAGT1656.9118905E-613.6361236
GATCATC2002.4875408E-713.50137211
GTAGGGC1000.00755043113.50029853
GTCAGGG1000.00755043113.50029852
AGGGCTC1000.00755241713.4997625
CTAGGTA1000.00755241713.4997624
Produced by FastQC (version 0.11.2)