FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_otd-1.352000000e8111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_otd-1.352000000e8111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867552
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC92630.23950550632544826No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG79980.20679747809467072No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG71190.1840699233003202No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT69520.1797519464508816No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG42770.1105867484134667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC5300.014.4332527
CTAGAGG5500.014.3176643
TGGACCG2551.3278623E-1014.1169545
TATACAC90950.013.8279945
GTGTAAG10750.013.8134541
GGACCGT2359.049472E-913.4035966
CCTCTAG5250.013.2852331
GCGACCT4700.012.44764523
TATACAG9250.012.4047975
CGACCTC4900.012.39879224
GTATTAG12500.011.879571
GACCTCG4950.011.81882325
GTTCTAG3451.05501385E-1011.7387071
TAGACTA5450.011.5596629
ACAGATC8400.011.5178878
TACACAG7500.011.399445
TAGGACG3957.2759576E-1211.3918465
TACACAT47250.011.33248345
ATACACA67600.011.04946745
GTATTAC9600.011.0152251
Produced by FastQC (version 0.11.2)