FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_obhd-3.352000000e81b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_obhd-3.352000000e81b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105179
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC89200.2872620225758322No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG79230.255154372743085No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG73520.23676573878671728No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT72160.23238595907031445No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG41700.1342917751279395No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA37740.12153888713017832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG6900.013.0434415
CCTATAC3405.456968E-1212.5734933
CTACACT5750.011.7390984
TCTAGGC3306.2937033E-1011.5908763
GTCTTAG2758.092866E-811.4545141
GTATAGG3358.076313E-1011.4178781
CAGGGCT4303.6379788E-1210.988344
CCTAGGA2707.886629E-710.83330252
GTACTAG3959.822543E-1110.8227541
GTTCTAG3754.5474735E-1010.799971
GTCTTAC3359.815267E-910.7462381
GTATTAC5900.010.6779361
GTCCTAT2554.6837304E-610.5882051
CTAGTAG2352.2023303E-510.5318853
TATACAG7500.010.499975
GTGCTAG3002.691104E-710.49996951
GGGTAGA4152.419256E-1010.3011751
TAAGACC4406.548362E-1110.2272434
GTCCTAA7100.010.1408161
TTATACA57100.010.12677845
Produced by FastQC (version 0.11.2)