FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_obhd-2.352000000e8198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_obhd-2.352000000e8198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3731380
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC88370.23682926959998712No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG76810.20584877444805944No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG72080.19317249918260804No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT72040.19306530023744567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG6450.017.7904855
CCTAGAC1859.632822E-814.59441953
GCCCTAG1453.1083837E-513.9653511
ACACCGG6000.013.8748356
GTCTAGG2053.3700417E-713.1705751
TTACACC8600.012.8196144
CCTAGAG7050.012.76666223
GTCCTAG2309.858741E-812.7172391
GTGTAAG10700.012.6166721
CCCTAGA2502.1498636E-812.5998512
CTTACAC10400.012.5479263
TCCTAGA7250.012.41447722
CCTCTAG4750.012.3156431
GGCTTAC8000.012.0936061
TACACAT30350.011.93544845
TAGGACG4550.011.86799055
CTAGGTA2301.3574136E-611.738994
TATACAC54900.011.72101545
TAGACTA5400.011.6666859
TCTAACC6950.011.6545383
Produced by FastQC (version 0.11.2)