FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_obhd-1.352000000e817e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_obhd-1.352000000e817e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3218376
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC136480.4240648078409732No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG117650.3655570387052352No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT114130.35461984553700376No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG112100.3483123165223703No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG55520.17250936497165031No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA49480.1537421357852532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT5450.013.2097874
GTGTAAG7750.013.063511
TACACCG4000.012.9363045
TATACAC65300.012.74747445
CCTCTAG5000.012.59903051
TATACTG8050.012.5764765
ATAGTGC5750.012.5211648
TACACAT34200.012.49864945
GTATTAG10150.012.4128381
TAGGACT4500.011.9988894
AGTACCG3052.2610038E-911.8021865
GTGTTAG5150.011.7952081
ATACACA50900.011.40348345
GTACAGG4051.2732926E-1111.1102551
GTACAGA4900.011.0195591
CTTACAC8300.010.8425393
CTAACCT5450.010.7329524
GCGACCT4651.8189894E-1210.64665823
CTAGGCC1700.001840410810.58725554
TACACTG6250.010.43903355
Produced by FastQC (version 0.11.2)