FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_bpbcd-3.352000000e8104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_bpbcd-3.352000000e8104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727728
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT5800.018.231845
TATACAC101050.015.18475345
CTGACGC7500.014.69922744
GTGTTAG5350.014.2993631
ATACACA71650.013.97344645
TACACAT44650.013.85705545
TTATACA147850.013.7716845
TACACCG2957.2759576E-1213.72884755
CTAGAGG5050.012.92106153
CTAGACA6050.012.6448914
TAGACAG7400.012.4662475
ACCGCGT1454.852208E-412.4138248
TCTGACG8550.012.36777143
CCTAGAC3900.012.1156363
CTACACT4400.011.7616084
GTAGCGT1350.004009423311.6666966
TATACAG6250.011.5200285
GTGTAAG6550.011.3361131
CCTCTAG4600.011.2502331
TAACCGA2201.06077805E-511.2500286
Produced by FastQC (version 0.11.2)