FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_bpbcd-2.352000000e80e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_bpbcd-2.352000000e80e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776468
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC81900.21686930751167494No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG71200.1885359547598444No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT66730.17669949804950022No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG65110.17240977548333522No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG43600.11545179252147775No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA37980.10057016238453496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG6750.013.9999273
GCGACCT7150.013.84681323
TACACAG9950.013.7935315
TGCGACC7700.013.442222
TAGGACG5100.013.2348745
TACACCG5950.012.8567345
TATACAC61050.012.78812145
ACACCGG6200.012.7012096
CGACCTC8000.012.3755924
CCGTGCG2756.153641E-912.2726619
GAGACCC5750.012.13069226
GGAACCT6700.012.0894888
GCTATAG16400.012.0731071
TAGGACT4850.012.0616324
TTGCGAC8500.011.91233221
TACCACG5500.011.8631027
TACACAT34900.011.8619345
CCGTTAG7050.011.8079841
TAGACTA4800.011.7186889
CGGAGTA5600.011.65203212
Produced by FastQC (version 0.11.2)