FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_bpbcd-1.352000000e80cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_bpbcd-1.352000000e80cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492942
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGAGG5100.017.6461943
CGATAAT5450.016.10206625
ATGGACC5550.015.81003637
AACCGAG3500.015.4275057
CCTCTAG4400.015.3401571
TAGACTA4200.015.0004689
CTAGAAC1951.1294105E-814.9992663
GATAGAC4200.014.9989647
TAGGACG3650.014.7934995
GGAACCT6050.014.5040038
ATCCACG6250.014.39987230
GTCCTAG1251.3820533E-414.3992931
GATCAGA7050.014.36215111
TACACCG3800.014.2095445
AGTACCG1605.0354283E-614.0615285
GTAGTAC1453.1086565E-513.9648323
GTACCGA1453.1092437E-513.9645526
TAGTACG3550.013.94241845
AGGAACC6150.013.9014797
TTTAGAC1301.9283523E-413.8454753
Produced by FastQC (version 0.11.2)