FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_bcd-3.352000000e80a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_bcd-3.352000000e80a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378182
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG6600.014.3184185
CTAGGTA2703.2923708E-1013.3334014
ACCGCGT1902.0654934E-613.0260858
GGACATC7100.012.9927277
CCTACAC8850.012.96661213
CGTACAC2451.6209015E-812.8572093
AGGACTG4750.012.7896845
GGACCGT1607.831446E-512.6563156
CTAGAGG6650.012.5188613
GACATCC7400.012.1619468
CGACCGT1300.003023458212.11558410
GAGACCC5600.011.65224426
ACACCGG5600.011.6518466
GTCTAGG1751.8065938E-411.5714861
CTTACAC13250.011.5472293
GGAACCT6350.011.3383818
CGACCTC5000.011.250442524
TAGGACT6650.011.1654714
ACAGTAC7700.011.1036998
CCTCTAG6100.011.065631
Produced by FastQC (version 0.11.2)