FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_bcd-2.352000000e8099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_bcd-2.352000000e8099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012645
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC30680.10183742193321815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC52950.014.27693145
CCTAGAC1605.0334547E-614.0622863
GTCCTAG1301.9280076E-413.8459441
GTTCTAG3001.2914825E-1012.7498071
GTATAGG5000.012.5998111
ACACCGT3102.2191671E-1012.3381146
TACACCG4600.012.2278745
GTACTAG5400.012.0831511
GTCCAGG5300.011.8866121
GTCTTAG2706.2751496E-811.666491
TGAGGAC4250.011.64746310
TAGGACT4700.011.4889974
TACACTG4350.011.3789485
CTAGAGG4950.011.3634653
TTATACA75550.011.34624245
TAGACTG3804.7293724E-1111.2496435
GGACCGG2402.266046E-611.2494566
TACACAT26900.011.20763745
GTATTAC7900.011.1074271
ATACACA41800.011.0879845
Produced by FastQC (version 0.11.2)