FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n02_bcd-1.352000000e807f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n02_bcd-1.352000000e807f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525659
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA1004.1702445E-415.7496331
TATACAC52950.014.19146145
CTAGAGG4650.014.0319323
TACACCG4000.013.4996855
TACCACG3900.013.2683967
AGGAACC4750.013.2623237
CGGAGTA3450.013.04420812
GCGACCT3850.012.85811623
CCTAAGG2805.857146E-1012.8568432
CCTCTAG3700.012.7699731
GGACATC4250.012.7050837
GTCTAAC3900.012.6920132
TCCGAAC1651.04408056E-412.2724413
CTTACAC7550.012.2182583
CTAGCAC2401.71598E-712.1872163
TTATACA75650.012.07434945
TTAGGCC2251.0409094E-611.9997224
TAGACTA3803.6379788E-1211.8425329
GGAACCT5150.011.7965438
TACACTG3254.8748916E-1011.7689565
Produced by FastQC (version 0.11.2)