FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_yw3.351000000e8059.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_yw3.351000000e8059.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4386232
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG110380.25165107545610904No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC106800.24348917248335245No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG98550.22468031786736314No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC97260.22173929696377212No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT85030.19385659490879645No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA50840.11590814165780561No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG48670.11096084292850904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTA15350.031.07506824
CGAGACT15350.031.07506823
CCACGAG16050.029.99945820
TAAGGCG16550.029.2290829
ACGAGAC16500.029.1819622
GACTAAG16650.029.05419326
TATGCCG16450.028.85966343
CTCGTAT16350.028.76095239
TCTCGTA16700.028.29290438
TCGTATG16800.028.12449540
TGCCGTC16950.028.00834745
CGAATCT16750.027.93979634
GCGAATC16900.027.82494433
ACTAAGG17550.027.56423427
GTATGCC17150.027.55052842
CGAGCCC17950.026.8240315
CCGAGCC18050.026.80007214
CGTATGC17800.026.54446641
AATCTCG17500.026.48523736
ATGCCGT18050.026.30146644