FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_yw2.351000000e803f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_yw2.351000000e803f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848195
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT3400.028.45526323
GACTAAG3400.028.45526326
GAGACTA3700.027.36426724
ATGCCGT3700.026.75617244
TCTCGTA4050.025.55499838
CTCGTAT3950.025.06274439
CGAGCCC3900.024.8071515
TAAGGCG3900.024.8071529
CGTATGC4100.024.69458241
TCGTATG4150.024.39705740
CCACGAG4150.023.854920
ACGAGAC4100.023.59704622
TATGCCG4150.023.31274243
GTATGCC4450.023.25791742
GCCCACG4500.021.4995318
CGAATCT4500.021.4995334
TGCCGTC4500.021.4995345
TCCGAGC4550.021.26327113
CCGAGCC4650.020.80599614
GCGAATC4650.020.32213633
Produced by FastQC (version 0.11.2)