FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_yw1.351000000e8015.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_yw1.351000000e8015.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2952349
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG63110.21376199087573994No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC61380.20790225003886734No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC59900.2028892925599243No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG56920.19279563493340388No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT47540.16102432334388653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAG8350.034.22208826
GAGACTA8400.033.75052324
CGAGACT9250.031.13561423
TAAGGCG9350.030.56093229
ACGAGAC9400.030.16004222
GCGAATC10000.028.34947633
CCACGAG10400.027.90813620
TATGCCG10050.027.76068143
CGAGCCC10350.027.39079915
TCTCGTA10300.027.3053238
GAATCTC10400.027.04276835
CGAATCT10800.026.45784634
CTCGTAT10650.026.40796539
ATGCCGT10600.026.3202744
CCGAGCC10950.026.0954114
CGTATGC10800.026.04118741
GCCCACG11200.025.91470118
CACGAGA11150.025.8291221
ACTAAGG10900.025.8031527
GTATGCC10900.025.59585842
Produced by FastQC (version 0.11.2)