FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_otd-2.351000000e813e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_otd-2.351000000e813e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257542
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC39990.3180013073122011No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC18710.14878230707205006No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG18590.14782806458949282No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG16700.13279874548921627No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT15080.1199164719746935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC5750.034.43414342
CGAGACT5750.034.43414323
TATGCCG6150.032.19452343
TCTCGTA6250.032.03940638
AGACTCC6200.031.93489325
CGAGCCC6150.031.82867615
CCACGAG6200.031.57199520
ACGAGAC6450.031.39476822
ATGCCGT6350.031.18052544
GCCCACG6400.030.93692618
GAGACTC6400.030.93692624
CACGAGA6450.030.69710521
TCGTATG6500.030.46097440
CTCGTAT6600.029.99944339
CCCACGA6600.029.99944319
CCGAGCC6550.029.88494314
TGCCGTC6650.029.77388245
CGTATGC6850.029.23303241
CCTGAGC6900.029.021230
GAGCCCA6750.028.9994616
Produced by FastQC (version 0.11.2)