FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_otd-1.351000000e8114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_otd-1.351000000e8114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867552
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC215830.5580532595295422No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG87530.22631887043794108No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC84400.21822589586384358No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG75250.19456751971272784No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT64770.16747027577134063No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA41830.10815627042635755No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG39170.10127853484581462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT26750.037.59768723
TGCCGTC27000.037.08289745
AGACTCC27800.036.1776325
CCACGAG27750.035.99957720
GAGACTC28050.035.6145524
TATGCCG28000.035.59779743
CGTATGC28100.035.5511841
TCTCGTA28100.035.5511838
CTCGTAT28200.035.42511439
ATGCCGT28200.035.42511444
GTATGCC28250.035.36241542
TCGTATG28450.035.1929140
CGAGCCC28600.034.92965715
GCCCACG28650.034.868718
ACGAGAC28950.034.58508722
TCCTGAG29250.034.23036629
GCATCTC29200.034.13487235
CCTGAGC29950.033.355230
GACTCCT30350.033.28626326
CCGAGCC30450.033.0291714