FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_obhd-3.351000000e81b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_obhd-3.351000000e81b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3105179
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG83560.2690988184578087No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC81730.2632054383982373No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG80900.26053248460072675No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT69670.22436709767778285No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA41090.13232731510808235No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG39590.127496675714991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAG2053.6379788E-1217.5708541
TCGTATG6450.016.74389340
ACGGACT6400.016.52341327
ACTCCTA6800.014.88944531
CCACGAG7100.014.57720920
GTATGCC7250.014.27561142
TATGCCG7300.013.86961943
GACGGAC7500.013.7999826
CGTATGC7500.013.79975941
CGAGCCC7700.013.73352515
ACGAGAC7950.013.58488722
CCTATCT8600.013.34278934
CCGAGCC8100.013.33309914
CACGAGA7950.013.30165421
TCTCGTA8050.013.136415538
CTCGTAT7950.013.0186439
AGACGGA8150.012.97544225
CGGACTC8050.012.85712428
CCCACGA8450.012.78084119
TGCCGTC8100.012.77755445
Produced by FastQC (version 0.11.2)