FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_obhd-2.351000000e819b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_obhd-2.351000000e819b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3731380
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG84010.22514458457728775No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC80880.21675626711833157No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG78510.21040472961746057No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT65360.17516307639532827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG5950.018.5295285
GACGGAC4400.017.3862426
GGGAGAC5350.015.981194524
ACGAGAC4800.014.53114522
ACACCGG4850.014.3815336
ACGGACT4950.014.09080827
GAGACCC6200.013.79022426
GCGACCT5100.013.67637323
TATACTG9800.013.5460035
ACCGCGT2501.4861143E-913.49972258
GGAGACC6550.013.3968525
TCTCGTA5400.013.3330638
TTAGACC7450.012.986318
AGACGGA5900.012.96600925
AGACCCT7300.012.94511227
CCTAGGC2104.515132E-712.85722353
GAGACGG5800.012.80163324
ACACCGT3700.012.77034956
CGAGCCC6000.012.74973915
GTATTAG12400.012.7100381
Produced by FastQC (version 0.11.2)