FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_obhd-1.351000000e8171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_obhd-1.351000000e8171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3218376
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG129900.4036197137935406No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG123360.3832989060321106No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC123300.38311247660310666No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT102690.3190739677402516No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA54400.16902934896357666No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG50910.1581853705098472No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC35660.11080122397134455RNA PCR Primer, Index 27 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAG7850.021.49643720
TCCTATC8300.021.14420533
GAGACGG8100.021.1107424
CCTATCT8400.020.8924934
CGAGCCC8500.020.64669415
TATCTCG8300.020.60204736
TCTCGTA8100.020.55519538
ACGGACT8300.020.33096727
GACGGAC8500.020.11729226
AGACGGA8650.019.76843825
CCGAGCC9050.019.64053714
ACTCCTA8750.019.54251331
TCCGAGC9100.019.53262313
GCCCACG9200.019.32031218
TATGCCG8900.019.21314443
GACTCCT9100.019.03812630
ACGAGAC9000.018.7496722
CGTATGC9250.018.72939941
CTCGTAT9050.018.39746739
TGCCGTC9100.018.29638145
Produced by FastQC (version 0.11.2)