FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_bpbcd-3.351000000e8107.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_bpbcd-3.351000000e8107.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2727728
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGC6150.017.56057445
TATACAC100950.015.22253545
TACGCCG1251.3811745E-414.4004635
ATACACA72200.013.89857445
TTATACA148200.013.80029245
TACACAT46300.013.31503145
GTCTTAG3450.013.0513151
TAAACCG1607.82736E-512.6566585
CTCCGAG8850.012.45734344
TAGACAG7350.012.2452925
TAGCACA5800.012.0262494
ACCGTTC1902.9514787E-511.8418368
CGTATAC2106.32055E-611.7860943
CTACACG1350.00400853211.6670434
TGTACCG2706.2700565E-811.6670435
CTACCCG1350.00401017911.6664019
ATACACG1751.8056606E-411.5718014
TAGGACG2158.213474E-611.5119995
TACACCG2758.087409E-811.4549145
TCTCCGA9550.011.07304643
Produced by FastQC (version 0.11.2)