FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_bpbcd-2.351000000e80ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_bpbcd-2.351000000e80ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776468
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG77400.20495341149454993No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC74320.19679764266505106No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG73080.19351415131810995No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT60670.1606527580797719No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA41990.1111885497242397No Hit
GTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTGTCTTG41260.10925552659257275No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC37930.10043776353990023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACA7450.021.14105223
CGTATGC8300.019.78873641
TCTCGTA8400.019.55315638
TCGTATG8150.019.32474340
CTCGTAT8600.019.09843339
CCACGAG8450.018.63865920
TATGCCG8500.018.26431843
CGAGCCC8700.017.8444515
ACGAGAC8900.017.69672222
GTATGCC9200.017.36376442
AATCTCG9500.016.81543236
CCGAGCC9650.016.7872114
GACAGGC9500.016.57903526
TGCCGTC9950.016.50718745
TCCGAGC9850.016.2179313
ATGCCGT10000.016.19965744
GCCCACG9850.015.98950918
CTAGAGG6300.015.7143693
AGACAGG10050.015.44784425
CTCCGAG10500.015.42824412
Produced by FastQC (version 0.11.2)