FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_bpbcd-1.351000000e80c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_bpbcd-1.351000000e80c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492942
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC26020.10437467056995309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC6250.023.04018422
CGAGACA6350.023.03168123
CCACGAG7700.019.57768620
CTCGTAT7450.019.32862739
TCCGAGC8000.019.12477113
AGACAGG7650.019.117825
GCCCACG7700.018.99327918
GACAGGC8400.018.75014926
CGAGCCC8350.018.32313315
CTAGAGG4500.017.499793
TCTCGTA8250.017.45433438
TCGTATG8250.017.45433440
TATGCCG8300.017.3491943
AATCTCG8250.017.18161236
ACAGGCA8950.017.0951127
GAGACAG8500.016.94131324
CCGAGCC9350.016.60407814
CACGAGA9150.016.22931321
CTCCGAG9450.016.19028312
CGTATGC9150.015.98341441
Produced by FastQC (version 0.11.2)