FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_bcd-3.351000000e80a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_bcd-3.351000000e80a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4378182
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC6800.028.12511427
GACCGTA6600.027.95465926
GAGACCG6950.027.51809924
AGACCGT6800.027.13246325
ACGAGAC7350.025.10214222
CGAGCCC7900.023.63879415
CCACGAG8000.023.06206720
CGTATGC8400.022.49957741
CGAGACC8550.022.36851323
TGCCGTC8350.022.3648545
TCGTATG8800.021.98822440
CCGAGCC8600.021.97633214
CTCGTAT8300.021.95741839
TCCGAGC8850.021.60976413
TATGCCG9300.021.53185543
TCTCGTA8400.021.16031638
GATCTCG8700.020.94788236
GTATGCC9300.020.5641342
CGTACTA9150.020.40945429
ATGCCGT9450.020.23771544
Produced by FastQC (version 0.11.2)