FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_bcd-2.351000000e809c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_bcd-2.351000000e809c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012645
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC3100.014.5231681
GGTCTAG1553.6036872E-614.5231681
TATACAC55800.013.46756445
CTAGCAC3051.2732926E-1113.2787323
ACCGTAC2754.4019544E-1013.090734527
CTGTACG3450.013.0433059
TATACTG9550.012.7225555
CTAGAGG4250.012.7059253
ACAGTCC2756.151822E-912.2725638
CTAAGAC2654.8390575E-811.8868323
GACCGTA2851.0450094E-811.841948526
CCCCGTA1902.9514316E-511.8419487
TGTACGA3306.2937033E-1011.59075510
TCCTACG2951.7396815E-811.4409062
CTAGAGC4151.8189894E-1211.385583
GTCTAGA2201.0552145E-511.2554551
GTCCTAT3256.0681487E-911.08229351
AGACCGT3052.8459908E-811.06542725
TGGACCG2656.196515E-711.0377735
TTATACA78300.010.9481345
Produced by FastQC (version 0.11.2)