FastQCFastQC Report
Thu 14 Jun 2018
HJV3MBCX2_l01n01_bcd-1.351000000e8072.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV3MBCX2_l01n01_bcd-1.351000000e8072.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525659
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC177620.7032620001354103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTA23450.038.4753626
TGCCGTC23800.037.908845
AGACCGT23950.037.8600125
ACCGTAC24200.037.3759227
ACGAGAC24350.037.05327222
ATGCCGT24700.036.52750844
GTATGCC24900.036.41483342
CGTACTA24950.036.34185429
CCACGAG24800.036.1987720
TATGCCG25100.036.03503443
GAGACCG25000.035.9998924
TCTCGTA24950.035.8909638
CGTATGC25150.035.87392841
CTCGTAT25150.035.87392839
TCCGAGC25150.035.87392813
TCGTATG25350.035.7684140
CGAGCCC25250.035.73185715
CCGAGCC25450.035.53946314
GCCCACG25350.035.50214418
CGAGACC25800.034.88361423
Produced by FastQC (version 0.11.2)