FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n02_atac_root_rep3_1.352000000ea291.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n02_atac_root_rep3_1.352000000ea291.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28380872
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC610750.022.85253745
TGACGCT263550.020.8435145
TCTGACG814000.018.64210345
ATCTGAC1010600.015.015161544
CATCTGA1144900.013.24597943
GTTCTAG10850.012.6466331
GTCCTAG9000.012.49691
GTGTAAG14150.012.3997521
GGGTAAG24350.012.101721
GTGTTAG9000.011.9970241
ACATCTG1284600.011.87770542
CTATACT11050.011.6034564
GTGTAGA42700.011.5896311
GTCTTAC14900.011.3226951
CTAGACT8100.011.1083554
TATACTG12100.010.9685475
GGTCTAG9450.010.9496651
TACACCG98550.010.8194245
CACATCT1407950.010.78047341
GTGCTAG11500.010.7582011
Produced by FastQC (version 0.11.2)