FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n02_atac_root_rep2_2.352000000ea2ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n02_atac_root_rep2_2.352000000ea2ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24614379
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC548700.023.44425845
TGACGCT240300.021.20322245
TCTGACG745000.018.5719245
ATCTGAC928250.014.91731344
CTAGACT6700.013.76472954
TAGACTG8000.013.4964245
CATCTGA1049300.013.1985343
GTATTAG7300.012.9415131
TAAGACT6400.012.6526394
GTCTTAC13200.012.6100391
TATACTG11300.012.1427995
GTGTAGA35500.011.9120941
GTCCTAC20850.011.8671171
ACATCTG1179500.011.79361942
GTCTAAG7450.011.7751641
GTATAAG12500.011.6966621
TAAGGGT10400.011.4630764
GTGCTAG11400.011.2467911
TACACCG66750.011.1880465
GTCCTAG7450.011.1713091
Produced by FastQC (version 0.11.2)