FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n02_atac_root_rep2_1.352000000ea277.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n02_atac_root_rep2_1.352000000ea277.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31083947
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT368300.025.1505545
CTGACGC898700.024.9212345
TCTGACG1267900.018.34067345
ATCTGAC1577050.014.75644744
CATCTGA1777800.013.07632443
GTACTAG5400.012.4969381
GTCCTAG8100.012.4969371
GTCTAGA7350.012.2418991
ACATCTG1990300.011.73475142
GTCTTAG8450.011.7131061
GTCTAGT6800.011.2472441
GTGCTAC25650.011.0499241
TTAGGAC7750.011.0295543
TAGACTG10000.011.0225295
CACATCT2148650.010.82883541
GGGTAAG21100.010.6608951
GTGTAGA46400.010.617011
GTGTAAG14850.010.6034621
TACACCG87600.010.3230295
GGTCTAG9000.010.2474881
Produced by FastQC (version 0.11.2)