FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n02_atac_root_rep1_1.352000000ea25e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n02_atac_root_rep1_1.352000000ea25e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26431386
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACGC532000.022.29316745
TGACGCT243350.019.84591745
TCTGACG689450.018.30861545
ATCTGAC852250.014.81112244
CATCTGA962600.013.11323643
GTCCTAG7950.012.16667651
GTGTTAG10000.012.1468711
ACATCTG1096300.011.57995542
CTACACT12300.011.5214214
GTCCTAC24500.011.017571
TACACCG85500.010.9973545
GTCTAGT7250.010.8592721
GTGTTAC14200.010.7718721
GTGTAGG41900.010.7370911
GTGTAAG14000.010.604411
CACATCT1195400.010.58503541
GTCTAGA7700.010.5167711
GTCTTAC14350.010.5025211
GGGTAAG20000.010.3473341
CTAGACT8050.010.3389514
Produced by FastQC (version 0.11.2)