FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n01_atac_root_rep3_1.351000000ea294.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n01_atac_root_rep3_1.351000000ea294.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28380872
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG508150.027.38327445
TCCGAGC210900.025.84076545
TCTCCGA848050.017.970845
ATCTCCG1059950.014.4049144
GTCCTAG8000.013.5058551
GTGTAAG14350.013.1764441
TAGGACT12300.012.8049024
CATCTCC1212900.012.59583443
GTCTAGA8750.012.348211
ACATCTC1277650.011.93455142
TAAGACT8400.011.7857374
GTATAAG13750.011.623221
GTGTAGA40100.011.5636171
TAAGGGT10400.011.4663684
GTATAGA17350.011.4170531
GTGTAAC16700.011.1874861
GTATAGG17300.011.0597081
TACACCG94650.010.9825895
GTCCTAC23650.010.9455491
CACATCT1409900.010.80726941
Produced by FastQC (version 0.11.2)