FastQCFastQC Report
Sat 23 Jun 2018
HJV33BCX2_l02n01_atac_root_rep2_2.351000000ea2bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJV33BCX2_l02n01_atac_root_rep2_2.351000000ea2bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24614379
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAG466300.027.3845945
TCCGAGC189350.027.08225445
TCTCCGA774600.017.9202145
ATCTCCG971950.014.28758844
GTGTAAG14050.012.9770771
TAGACTG8350.012.9341695
CATCTCC1110350.012.54318643
GTCTAGA7350.012.2501341
GTATAGA13850.012.0268011
CTAGACT5850.011.923114
GTATTAG7000.011.8979421
ACATCTC1170150.011.85404542
GTACAGA15550.011.7252681
GTGTAGG39150.011.6716561
GTGTAAC15000.011.4048741
TACACCG66050.011.3777765
GTGCTAG10250.011.1999091
GTGTAGA39900.011.1137721
CACATCT1282950.010.81198641
GTCCTAC21950.010.76771
Produced by FastQC (version 0.11.2)